LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
FIFTH SEMESTER – APRIL 2012
PB 5408 – BIOPHYSICS
Date : 30-04-2012 Dept. No. Max. : 100 Marks
Time : 1:00 – 4:00
PART A
Answer the following, each answer within 50 words: (10 x 2 = 20 marks)
- Define Brownian movement.
- What is sedimentation?
- Define osmosis.
- What is a buffer?
- Mention any two properties of colloids.
- What is isoelectric focusing?
- Define Beer-Lamberts law.
- What is fluorescence?
- Define radioactivity.
- What is free energy?
PART B
Answer the following, each answer within 500 words:
Draw diagrams wherever necessary: (5 x 7= 35 marks)
- Write notes on sedimentation and factors influencing it.
(or)
- Give a brief account of the kinetic theory of gases.
- Explain in brief about osmosis and factors affecting osmosis.
(or)
- Write notes on the various methods for preparing solutions of different concentration.
- Give an account of colloids, their properties and classifications.
(or)
- Write short notes on the principles of chromatography.
- Explain briefly about the properties of light.
(or)
- Discuss in brief about the fluorescence microscopy and its applications.
.
- Write short notes on radioactive decay.
(or)
- What are the various laws of thermodynamics?
PART C
Answer any three of the following, each answer within 1200 words:
Draw diagrams wherever necessary: (3 x 15= 45 marks)
- Discuss in detail about the principles and types of centrifugation.
- What is pH? Write detailed notes on the measurement of pH and the biological importance of pH.
- Explain in detail about various types of chromatography.
- Give a detailed explanation on the structure and functioning of scanning electron microscopes.
- What are radioisotopes? Explain in detail about the applications of radioisotopes in biology.
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